Vol. 9(2), pp. 16-26. November, 2021. 

ISSN: 2384-616X

https://doi.org/10.14662/arjb2021025

 

Full Length Research

 

Abstract

Medicinal plants are rich in phytochemical constituents that have been used by man as therapeutic agents in the treatment of several inflammatory-related diseases. Anogeissusleiocarpus DC. (Guill & Perr.) (African birch) belongs to the family of Combretaceae, it has been used in African traditional medicine particularly in South-western part of Nigeria to treat many illnesses including gastro-intestinal disorders, wounds, arthritis, malaria, diabetes, ulcer amongst others. Therefore, the present study is to evaluate the haematological profile and hepatoprotective potential of hexane and ethanolic stem bark extracts of Anogeissusleiocarpus in formalin induced pain and inflammation in wistar albino rats in other to validate folkloric claim of the study plant in the treatment of inflammation. Formalin-induced paw oedema model was used to investigate anti-inflammatory effects of hexane and ethanolicstem bark extract of Anogeissusleiocarpus in experimental rats. Liver tissue used to assay for biochemical parameters including Aspartate aminotransferase (AST), Alanine transaminase (ALT), Superoxidedismutase (SOD), Glutathione peroxidase (GPX), Malondialdehyde (MDA) and Nitric oxide (NO).Haematological parameters were also determinedusing an automatic hematology analyzer. There is a dearth of information on hepatoprotective potential of this study plant in pain and inflammation models induced by formalin in rats. The results of the study showed that formalin-induced pain in rats model produced reduction in the levels of total RBC and Hb and increase in the levels of total leucocytes and total WBC. However, daily oral administration of stem bark extract of Anogeissusleiocarpus for fourteen days significantly increased the levels of total RBC and H band caused reduction in levels of total leucocytes and total WBC. The formalin-induced pain and inflammation in the animal model also resulted in a significant (p<0.05) increase in AST, ALT, NO, MDA and significant (p<0.05) reduction in GPx, SOD and GSH tissue activities. However, the administration of hexane and ethanolic stem bark extracts of Anogeissusleiocarpus resulted in significant (p<0.05) reduction in tissue levels of ALP, AST, ALT, NO MDA and significant (p<0.05) increase in tissue activity of GPx, SOD and GSH activities in a dose dependent manner. Results obtained from this study revealed that hexane and ethanolic stem bark extracts of Anogeissusleiocarpus could be explored as a natural product in preventing oxidative stress mediated diseases and liver damage.

 

Keywords: Anogeissus leiocarpus, inflammation, haematology, hepatoprotective.

 

	Cite This Article As: Akande, T., Salawu, S.O., Akindahunsi, A.A., Mulinacci, N. ((2021). Haematological Profile and Hepato protective Property of Stem bark Extracts of Anogeissus leiocarpus against Formalin - Induced Pain and Inflammation in Wistar Rats. Acad. Res. J. Biotech. 9(2): 16-26